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Name :
Dr. Desouky A. M. Abd-El-Haleem
Marital status : married (3 Children)
Date of birth : 17. 5. 1969
Nationality : Egyptian
Current Address :
Environmental Biotechnology Department,
Genetic Engineering and Biotechnology Research Institute,
Mubarak City for Scientific Research and Technology Applications,
New Burg Al-Arab, Alexandria, Egypt .
Tel : 002-03-4594391 (Private);
: 002-012-4706152 (mobile)
Qualifications:
Academic studies :
• 1999, Ph.D.
Environmental Biotechnology & gene technology,
at Microbiology and Hygiene institute, Faculty of Medicine,
Humboldt University Berlin, Germany.
• 1995, M.Sc. at Faculty of Agriculture,
Cairo University.
• 1990, B.Sc. at Faculty of Agriculture
(Very good with honour),
Assiut University.
Linguistic : English, German and Arabic
Computer skill :
Work with basic computer programs (MS Office, Word, Excel, Power
Point, etc), Many statistical analysis and graphic programs etc.
Professional work:
Current position: Associate
Prof., Environmental Biotechnology Dept., Genetic Engineering and
Biotechnology Research Inst, Mubarak City for Scientific Research, New Burg
Al-Arab, Alexandria, Egypt.
Employment and Research Experience
• December 1991, Researcher Assistance at Botany department,
National Research Centre, Dokki, Cairo, Egypt.
• Augst 1995, Assistance Researcher at Botany department, National
Research Centre, Dokki, Cairo, Egypt.
• June 1996 – September 1996: Visitor at Institute for
Environmental protection, Technical University, Berlin, Germany (Nitrification
and denitrification in rhizoremediation system).
• From October 2000 to March 2001, Post-doc. In Centre for
Environmental biotechnology, University of Tennessee Knoxville, USA, (Construction
of genetically modified biosensors to detect environmental pollutants).
• From June 1996 to October 1999 Ph.D. student in Microbiology
and Hygiene Institute., Faculty of Medicine, Humboldt University, Berlin,
Germany.
• From December 1991 to August 1995, Researcher assistant in
Botany Dept., National Research Centre, Dokki, Cairo, Egypt.
• April 2000 – 2005, Senior Researcher,
Environmental Biotechnology Dept., Genetic Engineering and Biotechnology
Research Inst., Mubarak City for Scientific Research, New Burg Al-Arab,
Alexandria, Egypt.
• June 2005 – present, Associate Prof., Environmental
Biotechnology Dept., Genetic Engineering and Biotechnology Research Inst.,
Mubarak City for Scientific Research, New Burg Al-Arab, Alexandria, Egypt.
Teach Experience
• Central lecture at the Egyptian Ministry of Culture from
2001 to present.
• Conducting (teaching) several biotechnology, molecular Biology
and microbiology workshops at national and international levels.
General Experience:
• Direct microbial DNA extraction from soil/roots or water.
• PCR amplification using universal or specific primers, cloning,
Gene libraries, Screening of the gene libraries, sequencing, sequence analysis,
• Fluorescent In situ hybridization (FISH).
• The use of RFLP, REP-PCR and others PCR-based approaches
to recognize between bacterial species at the strain level.
• Probes and primers design.
• Screening and Constructing of genetically modified biopolymers
microbial-producers and analysis.
• Phylogenetic analysis of 16S ribosomal RNA genes either for
cultured or yet uncultured microbial species from different environmental
Ecosystems.
• Construction of genetically modified biosensors (Bioluminescent
reporter)
• Gene Expression through the use of differential display technique.
• Phytoremediation and biodegradation as tools for bioremediation
of organic and inorganic pollutants.
Main research Topics:
• Production of environmentally monitoring and diagnostic kits.
• Biodiversity and phylogenetic analysis of bacterial isolates
using ribosomal RNA as a genetic marker.
• Construction of genetically modified biosensors to monitor
pollutants in soil and water at molecular level.
• Production of biodegradable biopolymers from some Egyptian
microbial isolates.
• Microbial biodegradation of aliphatic, mono and poly aromatic
hydrocarbons.
Projects:
• Principle Investigator:
Genetically modified biosensor to detect nitrite in wastewater (US-Egypt
joint fund program) (2002) (Run)
• Principle Investigator:
Production of biopolymers from some Egyptian bacterial isolates. Funded
by GEBRI (Finished)
•
Member: Biological nitrogen fixation (member
of the teamwork) funded by European community (Finished)
• Principle Investigator: Production
of biopolymers In transgenic Yeasts Funded by Egyptian Academy of science
and technology (Run)
Supervisions
• Ph.D. Thesis with Mr. Elsayed
Hafez under title of “Production of biopolymer from some Egyptian
bacterial isolates) at Mansoura University (Finished 2003)
• Ph.D. Thesis with Maha Gaffer
under title of “Genotyping of microsporida isolated from stool of
immunocompromised patient” at Alexandria University (Finished 2003)
• M.Sc. Thesis with Walaa Salaheldin
under title of “Multiplex-PCR to detect some pathogenic bacteria in
water) at Ain Shams University (Finished 2005).
• M.Sc. With Mr. Gadallah Abou
Elreash " Genetically modified biosensor to monitor toxicants in wastewater"
at Faculty of Science, Alazhar University (Finished 2005).
• M.Sc. With Marwa Elfaham "
Detection of Schistosoma by PCR" at Faculty of medicine, Alexandria
University (Finished 2005).
• M.Sc. with Marwa Mustafa “
Detection of bacterial pathogens in water and wastewaters by PCR”
at faculty of Science Alexandria University. (run)
• M.Sc. With Mr. Mohamed Elsayed
“ Production of biopolymer from genetically modified bacterial strains”
at faculty of Science Alexandria University.
• Ph.D. With Salma Hashem “Employment
of bacterial Probiotic in poultry feeding ” at faculty of Agriculture,
Saba-Pasha, Alexandria University (run)
• Ph.D. With Gadallah Abou Elreash
" cyanide bioreporter " at Faculty of Science, Alazhar University
(run).
Honour awards and prizes
• Abdul Hamied Shoman Prize For Young Arab Researchers In “Biological
And Environmental Sciences 2004”.
• Encourage State Prize of Egypt in Advanced
Technology Serve the Agricultural Sciences, 2002.
• Distinguished scientific achievement
award for the year 2001-2002 from Mubarak City for Scientific Research and
Technology Applications.
• Medal of Mubarak City for Scientific
Research and Technology Applications 2002 and 2003.
• Honour shield of Mubarak City for Scientific
Research & Technology Applications 2002.
Editorial Boards
• Principal member in the editorial board
of African Journal of biotechnology from 2003 – present.
• Principal member in the editorial board
of Research Journal of Biological Sciences from 2005 – present.
• Principal member in the editorial board
of International Journal of Engineering Management and Sciences from 2005
– present.
• Chief Editor of journal “Developmental
Microbiology and Molecular Biology” from 2005 – present.
Scientific associations
• Arab association of biotechnology from
2003-present.
• Association of Environmental Arab experts
2004-present.
Conferences organization
• Associated member in the organization
committee of US-Egypt Genetic engineering and Genome workshop in the period
between 5-8 December 2003 at Mubarak City for Scientific Research and Technology
Applications, Alexandria, Egypt.
• Associated contributor in preparing
for the first National Conferences on Scientific Research plane till the
Year 2017 in the period between 28-29 May 2005.
Innovative Bio-Products
• BIO-FRIEND:
a bacterial consortium to treat sewage wastewater.
• AMSHAGE:
DNA extraction kit from bacteria, fungi, water, parasites and whole blood.
• PathoTrack:
PCR detection kit of Salmonella, E. coli and P. aeruginosa in biological
samples.
• NEBRAS:
Genomic DNA, PCR and Plasmid purification kit
Services for the work place and others
• Design and consult of several biological
waste water treatment units in the region of Alexandria and Matroh.
• Responsible for ordering and purchasing
general and fine chemicals for the Genetic Engineering and Biotechnology
Research Institute, Mubarak City from 2002-present.
• Member of the technical office in Mubarak
City for Scientific Research during the period 2001-2002.
Scientific publications
1-Conferences Oral and Poster presentations
1. Desouky Abd-El-Haleem, v. Wintzingerode
F., Hegemann W. and Goebel U. (1997): Phylogenetic analysis of ammonia oxidizing
bacteria occurring in the nitrifying sections of two root-zone method based
wastewater treatment plants. Kongress der Deutschen Geselshaft fuer Hygiene
und Mikrobiologie (DGHM), 5-9. 10. 1997, Jena, (Poster 483).
2. v. Wintzingerode F., Schloetelburg C., Desouky
Abd El-Haleem and Goebel U. (1998): A new rapid screening method for environmental
16S rDNA clone libraries. Methodische Entwickungen in der mikrobiologischen
Nukeinsaere-Diagnostik, Berlin, 24-25 April/1998. (Poster)
3. Desouky Abd El-Haleem, v. Wintzingerode
F., Moter A., Moawad H. and Goebel U. (2000): Molecular analysis of root-associated
Nitrobacter-like bacteria in a wastewater treatment plant-based rhizoremediation
technology. The second congress of genetic science at Faculty of agriculture,
El-Menia University, Menia, Egypt ,October 2000( Lecture 20 min).
4. Desouky Abd El-Haleem (2001) Molecular analysis
of root-associated nitrifying bacteria in a wastewater treatment plant based
rhizoremediation technology. First US-Egypt workshop in microbial ecology,
National Research Centre, Cairo, Egypt 6-10 May 2001 (Lecture 25 min).
5. Desouky Abd El-Haleem, Steve Ripp, and Gary
Sayler: A Bioluminescent Bioreporter for the Detection of Phenol. 9th ASM
Microbiology Education Conference, May 17-19, (2002). Salt Lake City, Utah,
Topic Q04 Biodegradation Heterocyclics and Aromatic Compounds.
6. Desouky Abd-El-Haleem, Elsayed Hafez, Mohamed
Nagieb, and Ahmed Eldewany (2002). Production of biopolymer from Egyptian
wild type and mutants bacterial strains. The Alexandria Conference on Biotechnology
and Sustainable Development: Voices of the South and North, March 16-20,
2002, at the Library of Alexandria, Egypt.
7. Desouky Abd-El-Haleem and Sahar Zaki. (2002):
Production of Poly(?-Glutamic Acid) in a novel Bacillus sp. strain S-5.
International symposium on biological polyesters. P. 77, September 22-26,
2002. Muenster, Germany.
8. Desouky Abd-El-Haleem (2003): Biomonitoring
of genetically modified organisms (GMOs). New era of biotechnology in pharmaceutical
industries, Mubarak City for Scientific Research and Technology Applications,
Alexandria, 22-23 September 2003 (Lecture 45 min) .
9. Sahar A. Zaki, Desouky Abd-El-Haleem, Hassan
Moawad and Mona Elsayed (2003): Application of PCR-RFLP, Rep-PCR and enzyme
assays to determine heterogeneity among phenol degraders. US-Egypt Workshop
on Genetic Engineering and Genomics, 5-8 December, 2003, Poster, at Mubarak
City for Scientific Research and Technology Applications, New Borg El-Arab,
Alexandria, Egypt.
10. Desouky Abd-El-Haleem, Sahar Zaki, Hany
Hussein, Hassan Moawad and Mona Elsayed (2003) Acceleration of Sewage Wastewater
Treatment Process Using Efficient Bacterial Inoculants. US-Egypt Workshop
on Genetic Engineering and Genomics, 5-8 December, 2003, Poster, at Mubarak
City for Scientific Research and Technology Applications, New Borg El-Arab,
Alexandria, Egypt.
11. Desouky Abd-El-Haleem, Mohamed Nagieb,
Salah Eldehlob, Ahmed Eldewany and Elsayed Hafez Isolation, Characterization
And Expression Of Phb Synthase Gene (Phac1) From Pseudomonas Reactans Strain
DS-4 US-Egypt Workshop on Genetic Engineering and Genomics, 5-8 December,
2003, Poster, at Mubarak City for Scientific Research and Technology Applications,
New Borg El-Arab, Alexandria, Egypt.
12. Desouky Abd-El-Haleem, Sahar Zaki, Gadallah
AbuElreesh and Mona Elsayed (2003) A Universal Bioluminescent Bioreporter
To Monitor Toxicants In Wastewater. US-Egypt Workshop on Genetic Engineering
and Genomics, 5-8 December, 2003, Lecture, at Mubarak City for Scientific
Research and Technology Applications, New Borg El-Arab, Alexandria, Egypt
(Lecture 20 min).
13. Desouky Abd-El-Haleem, Sahar Zaki and Gadallah
Abu Elreesh (2004) Toxicity Assessment of Pollutants Using Genetically Modified
Bioluminescent Bioreporter. Envi-Workshop On Liquid-Solid Wastes From Agricultural
and Industrial Sources, Alexandria University 14-20 March 2004 (Lecture
20 min).
14. Desouky Abd-El-Haleem and Hassan Moawad
(2004): Molecular Monitoring of Released Genetically Modified Microorganisms
Into The Environments. The third forum on the cooperation between Arab countries
in scientific research, Alryhad, Saudi Arabia, 11-17 April 2004 (Lecture
20 min).
15. Desouky Abd-El-Haleem, Sahar Zaki, Steven
Ripp and Gary Sayler (2004): Genetically modified bioluminescent reporter
to monitor nitrite toxicity. The Eighth world congress on biosensors, Gernada,
Spain, 24-26 May 2004.
2- Published Research Papers
1. Desouky Abd-El-Haleem, v. Wintzingerode
F., Moter A., Moawad H. and Goebel U. (2000): Phylogenetic analysis of rhizosphere-associated
beta-subclass ammonia oxidizers in a municipal wastewater treatment plant
based on the rhizoremediation technology. Letters in Applied Microbiology
30: 34-40.
2. Desouky Abd-El-Haleem, Alice Layton and
Gary Sayler (2002): Long-PCR amplified rDNA apply for PCR-RFLP and REP-PCR
based approaches to recognize closely related microbial species, Journal
of Microbiological Methods 49: 315-318.
3. Desouky Abd-El-Haleem, Hassan Moawad, Essam
A. Zaki and Sahar Zaki (2002): Molecular characterization of phenol-degrading
bacteria isolated from different Egyptian ecosystems. Microbial Ecology
43: 217-224
4. Usama Beshey, Desouky Abd El-Haleem, Hassan
Moawad and Sahar Zaki (2002): Phenol biodegradation by free and immobilized
Acinetobacter. Biotechnology letters 24: 1295-1297.
5. Desouky Abd El-Haleem, Steven Ripp, C Scott
and Gary Sayler (2002): A luxCDABE-Based Bioluminescent Bioreporter for
the Detection of Phenol. Journal of Industrial Microbiology and Biotechnology.
29: 233-237.
6. Desouky Abd El-Haleem, Usama Beshey, Abdou
Abdelhamid, Hassan Moawad and Sahar Zaki (2002): Effects of mixed nitrogen
sources on biodegradation of phenol by immobilized Acinetobacter sp. strain
W-17. African Journal of Biotechnology 2: 8-12.
7. Desouky Abd El-Haleem (2003): Acinetobacter:
Environmental and biotechnology applications. African Journal of Biotechnology,
2 (4), 71-74.
8. Eman El-Kerdany, Desouky Abd-El-Haleem (2003):
Genotypic discrimination between a Leishmania isolate and two Leishmania
major reference strains using PCR-RFLP technique. Journal of the Egyptian
society of Parasitology. 33: 261-273, April 2003.
9. Desouky Abd-El-Haleem, Sahar Zaki, Gadallah
Abu-Elreesh and Mona Elsayed (2003): An Universal Bioluminescent Bioreporter
To Monitor Toxicants In Wastewater. US-Egypt Workshop on Genetic Engineering
and Genomics, 5-8 December, 2003, Lecture, at Mubarak City for Scientific
Research and Technology Applications, New Borg El-Arab, Alexandria, Egypt
(Lecture 20 min).
10. Desouky Abd-El-Haleem, Zeinab Kheirallah,
Sahar Zaki and Walla Abdelrahiem (2003): Multiplex-PCR & PCR-RFLP assays
to monitor water quality against pathogenic bacteria. Journal of Environmental
Monitoring, 5: 865-870.
11. Azza Y Negm and Desouky Abd-El-Haleem (2004):
detection of Trichomoniasis in vaginal specimens by both conventional and
modern molecular tools. Journal of the Egyptian society of Parasitology.
34 (2): 589-600.
12. Hassan Moawad, Wafaa M. Abd El-Rahim and
Desouky Abd-El-Haleem (2004): Performance of phaseouls bean rhizobia in
soils from major production sites in the Nile Delta. Comptes Rendus Biologies,
327: 445-453.
13. Desouky Abd-El-Haleem and Hassan Moawad
(2004): Molecular Monitoring of Released Genetically Modified Microorganisms
Into The Environments. The third fourm on the cooperation between arab countries
in scientific research, Alryad, Saudi arabia, 11-17 April 2004.
14. Kashyout A.B., Soliman M., and Desouky
Abd-El-Haleem (2004) Disinfection of Bacterial Suspensions by Photocatalytic
Oxidation with TiO2 and Ultraviolet Light. World conference on energy for
sustainable development: technology advances & environmental issues.
6-9 December 2004.
15. Desouky Abd-El-Haleem (2004): Microbial
Biosensors: Principles and Applications. Arab Journal of Science and Information,
no. 3 , year 18, June 2004: 12-19.
16. Elrashdy Redwan and Desouky Abd-El-Haleem
(2005): Molecular analysis of cross-bacterial contamination detected during
diagnosis HCV infection. J. Appl. Sci. Environ. Mgt. 9: 5 – 10.
17. Hassan Moawad, Wafaa M. Abd El-Rahim and
Desouky Abd-El-Haleem (2005): Competition of phaseouls bean rhizobia in
soils from major production sites in the Nile Delta. Basic Journal of Microbiology
(In press).
18. Abeer A. Rushdy, Desouky Abd-El-Haleem,
Zeinab Kheirallah (2005): Effect of seasonal variations, microbial, physical
and chemical factors on the quality of irrigation water cultivated areas
at East Delta in Egypt (submitted).
19. Desouky Abd-El-Haleem and Sahar Zaki (2005):
Determination of antimicrobial activities in Bacillus cereus strain BAC(S5)
using bioreporter carrying luxCDABE genes (submitted).
20. Sahar Zaki, Desouky Abd-El-Haleem, Hassan
Elbery, Ashraf Tawfiek and Gadallah Abu-Elreesh (2005): Assessment of phenolics
toxicity using a constructed bioluminescent Acinetobacter bioreporter (submitted).
21. Desouky Abd-El-Haleem, Sahar Zaki, Hassan
Elbery, Ashraf Tawfiek and Gadallah Abu-Elreesh (2005): Assessment of Heavy
metals toxicity in wastewater using a constructed bioluminescent Acinetobacter
bioreporter (submitted).
Patents
1. Desouky Abd-El-Haleem, Sahar Zaki and Zeinab
Kheirallah (2003) Multiplex PCR to monitor water quality against pathogenic
bacteria [Egyptian patent office ASRT (Regist. Patent. No. 2003030256)].
2. Desouky Abd-El-Haleem, Elsayed Hafez (2003)
Production of bioplastic from a novel Egyptian bacterial strain [Egyptian
patent office ASRT (Regist. Patent. No. 2003040352)].
3. Desouky Abd-El-Haleem, Sahar Zaki and Hassan
Moawad (2003) Bacterial consortium to treat sewage wastewaters [Egyptian
patent office ASRT (Regist. Patent. No. 20031210278)].
4. Desouky Abd-El-Haleem, (2004) DNA extraction
from bacteria, fungi, waters and whole blood [Egyptian patent office ASRT
(Regist. Patent. No. 2004030147)].
5. Desouky Abd-El-Haleem, et al. (2004) Genetically
modified biosensor to monitor general toxicity in liquids [Egyptian patent
office ASRT (Regist. Patent. No. 2004030107)].
Books
1. Desouky Abd-El-Haleem, (in Arabic) Environmental
Biotechnology (2003) (Egyptian Academy of Science and Technology).
2. Desouky Abd-El-Haleem, (in Arabic) Friendly
plastics (2004) (Egyptian Academy of Science and Technology).
3. Desouky Abd-El-Haleem, (in Arabic) Secrets
of Water (2005) (in preparation)
